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PLASMIDS
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Required fields are in purple.
Name and aliases
Description
e.g., mouse kidney full Fut10
ORF, TA cloned
Insert description
e.g., mFut10 full ORF 1429 bp
GenBank ID BA12653
Vector
e.g., pcDNA3.1/Myc-His/B+
Generation
e.g., EcoRI-fragment from pCR2.1
clone blunt-cloned into BamHI-SpeI
vector fragment of pSecFc-mFut10
given by Santosh
Mammalian promoter
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CMV
EF1 alpha
H1
LTR
None
SV40
Tet
TK
U6
Other promoters
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SP6
T3
T7
Special features
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APRT
DHFR
Galactosidase
HGPRT
IRES
TK
Fusion tags
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C-terminal alk. phos.
C-terminal FLAG
C-terminal GFP
C-terminal GST
C-terminal hIgG1 Fc
C-terminal HA
C-terminal His
C-terminal Myc
C-terminal V5
N-terminal alk. phos.
N-terminal FLAG
N-terminal GFP
N-terminal GST
N-terminal HA
N-terminal hIgG1 Fc
N-terminal His
N-terminal Myc
N-terminal V5
Bacterial selection
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Actinomycin
Ampicillin
Carbenicillin
Cefotaxime
Chloramphenicol
Kanamycin
Streptomycin
Tetracycline
Other selection
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Blasticidin S
G418/genticin/neomycin
Hygromycin
Mycophenolic acid
None
Puromycin
Zeocin
Storage box
Storage box location
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3rd floor min 20
Fridge 1 at min 20
Fridge 2 at min 20
Fridge 3 at min 20
Lab REVCO -80
Storage box for bacteria
bacteria carrying the plasmid
Location of above box
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4th floor -135
4th floor liquid N2
C524 Harris -80
Lab REVCO -80
Source
e.g., self, Shaolin, Stein lab
Acquired on
optional; e.g., June 2001
Experiment info.
e.g., made in SS41
Comment
Plasmid map
Filename should have extension. Limit width to 700 px.
Insert sequence
Plain text (.txt) or Word (.doc) file will be best
Extra file
e.g., entire vector sequence. Filename should have extension. Zip if more than one file
Added by
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Barbara Salas
Carolina Valdivia
Claudia Valdes
Elena Vidal
Felipe Aceituno
Laboratorio
Pamela Naulin
Rodrigo Gutierrez
Modified by
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Barbara Salas
Carolina Valdivia
Claudia Valdes
Elena Vidal
Felipe Aceituno
Laboratorio
Pamela Naulin
Rodrigo Gutierrez
Owner
Choose one ...
Barbara Salas
Carolina Valdivia
Claudia Valdes
Common Lab
Elena Vidal
Felipe Aceituno
Laboratorio
Pamela Naulin
Rodrigo Gutierrez
Tatiana Kraiser
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